Copper Acetate Manufacturers(WSDTY) proposed real-time PCR was performed to determine the expression of 8 genes, which were involved in skin irritation and inflammation. These genes were previously found to be upregulated in the presence of irritants. The fold changes in relative gene expression of treatment cells compared to sterile water treated control cultures were copper acetate determined following 24?h of treatment.
From SI 1, it was shown that heat shock protein 27 (HSP27), superoxide dismutase 1 (SOD1), cofilin 1 (CFL1) and bone morphogenetic protein 2 (BMP2), did not show any significant change in expression levels among all the treatment groups with p>0.05. While the change in gene expression of the other four genes, interleukin 1 alpha (IL1A), interleukin 8 (IL8), FOS-like antigen 1 (FOSL1) and heat shock protein 70kDa 1A (HSPA1A) were significantly different among the control and treatment groups (p<0.05), which were further plotted.
For CuCl2 and copper acetate, the gene expression levels were concentration-dependent, while the treatment concentration of 580?μM induced a higher expression of these genes than that of 58?μM. At 58μM, CuCl2 and Cu(OAc)2 didn’t induce any significant upregulation of the four genes. After treating HaCaT with 580μM CuCl2 or Cu(OAc)2, the expression of IL1A, IL8, HSPA1A and FOSL1 were significantly higher than that in the control group and ranged from a 8–44 fold change. Specifically, 580μM CuCl2 induced the upregulation of IL8 up to 44 times. At the same concentration of 580μM, Cu(OAc)2 induced a higher upregulation of IL1A than CuCl2, while CuCl2 induced a higher upregulation of IL8, HSPA1A and FOSL1 than Cu(OAc)2.
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